Journal: International Journal of Molecular Sciences

Article Title: Mitochondrial Ribosomal Protein MRPS15 Is a Component of Cytosolic Ribosomes and Regulates Translation in Stressed Cardiomyocytes

doi: 10.3390/ijms25063250

Figure Lengend Snippet: Key resources list.

Article Snippet: Antibodies were diluted in blocking solution: rabbit anti-MRPS15 diluted 1:500, (Abcam, Ab137070), mouse anti-RPS7 diluted 1:100 (Santa Cruz, Sc377317), mouse anti-RPS13 diluted 1:50 (Santa Cruz, Sc-398690), before the addition of the cover slip, for 1 h at room temperature.

Techniques: Bacteria, Virus, Recombinant, Reverse Transcription, Plasmid Preparation, PCR Cloning, In Situ, Reporter Assay, Modification, Sequencing, Software, Microscopy, Western Blot

Journal: Genes & Diseases

Article Title: Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

doi: 10.1016/j.gendis.2022.07.002

Figure Lengend Snippet: BC-3 target proteins analyzed by mass spectrometry. ( A ) The binding of biotin- and FITC-labeled BC-3 to 5637 cells was measured by flow cytometry, with biotin- and FITC-labeled Library as a control. ( B ) SDS-PAGE with silver staining was used to identify the aptamer target. Lane library, protein captured with biotin-labeled library treated sample; Lane BC-3, protein captured with biotin-labeled BC-3 treated sample. ( C ) Western blot analysis of the concentrations of RPS7 and β-actin in the preparations captured via Library and BC-3. ( D ) After incubating the ER fluorescence probe ER-tracker (red) for 0.5 h and FITC-labeled BC-3 or Library (green) for 1 h, 5637 cells displayed the colocalization (yellow) of ER-tracker and FITC-labeled BC-3. ( E ) Enlarged view of intracellular fluorescence signals in colocalization. ( F ) Fluorescence intensity profile of yellow arrow regions in 5637 cells (scale bar = 40 μm).

Article Snippet: Firstly, the protein structure of RPS7 was downloaded from the RCSB PDB databank (PDB ID: 6YBW) with a resolution of 3.1 Å.

Techniques: Mass Spectrometry, Binding Assay, Labeling, Flow Cytometry, Control, SDS Page, Silver Staining, Western Blot, Fluorescence

Journal: Genes & Diseases

Article Title: Identification of new aptamer BC-3 targeting RPS7 from rapid screening for bladder carcinoma

doi: 10.1016/j.gendis.2022.07.002

Figure Lengend Snippet: Computational analysis of the Binding Model between RPS7 and BC-3. ( A ) Interaction model between RPS7 and BC-3. Close view of the RPS7 and BC-3 interaction region in part α and part β with key amino acid residues marked. Promoter methylation ( B ) and the expression level ( C ) of RPS7 in bladder cancer and normal bladder samples from TCGA.

Article Snippet: Firstly, the protein structure of RPS7 was downloaded from the RCSB PDB databank (PDB ID: 6YBW) with a resolution of 3.1 Å.

Techniques: Binding Assay, Methylation, Expressing